A method to quantify orofacial development will allow for more rigorous analysis of orofacial phenotypes upon abrogation with substances that can genetically or molecularly manipulate gene expression or protein function. Early development of many animals is characterized by rapid cleavages that dramatically decrease cell size, but how the mitotic spindle adapts to changing cell dimensions is not understood. The material that Xenopus can offer in biology is great in terms of quality and quantity. This chapter provides a practical guide to understanding and manipulating the early development of Xenopus laevis, the African clawed frog.It describes the structure of the embryo and the locations, movements, and changes in shape and position of the major prospective tissues through the blastula, gastrula, and neurula stages. Xenopus has become an important tool for dissecting the mechanisms governing craniofacial development and defects. The embryos develop externally, are large in size, can be produced in large numbers, and are easily manipulated through microinjection or surgical procedures. Contact publisher for permission to reproduce images. Peter Hausen and Metta Riebsell 1991 Verlag der Zeitschrift für Naturforschung ISBN: 0-387-53740-6. Download : Download video (987KB) Movie 1. To identify mechanisms that scale the spindle during Xenopus laevis embryogenesis, we established an in vitro … Images reproduced on Xenbase with permission from the publisher. Xenopus is one of the leading vertebrate models for the study of gene function in development. Xenopus laevis embryo extracts were generated by fertilizing eggs in vitro with crushed testes, dejellying with 3% cysteine in water pH 7.7, and allowing development to proceed at 23°C to a time point ∼15 min prior to the desired stage for extracts or fully … Click here to order your own pet xenopus from Xenopus One. Early development: X. laevis histology . The combination of lineage analysis, gene-knockout strategies, experimental manipulation of the embryo, and genomic/bioinformatic techniques, makes it ideal for studies on the molecular A video from an open-access article co-authored by Gurdon: Animal view of different embryos developing in Xenopus laevis eggs: a diploid laevis x laevis is shown on the top, cleaving and entering gastrulation about 50 min earlier than haploid [laevis] x laevis (middle) and [laevis] x … On June 20, 2008, Kelly, one of the owners, met me inside the facility and even gave me a … After some internet research, I found out that one of the largest xenopus distributors, Xenopus One, was right in Dexter, MI, only about 50 minutes away from me. 2D MRI time-lapse sequence showing the morphogenetic tissue movements of the Xenopus embryo from early blastula to about stage 10.5. Animation of Xenopus Gastrulation | This animation consists of 72 frames of drawings of median sections of the Xenopus laevis embryo (stacked and aligned images of the drawings constitute the movie), covering the period of gastrulation from stage 9 to stage 14, approximately 9 to 17 hours post fertilization of the egg, at room temperature (Nieuwkoop and Faber, 1967). The Early Development of Xenopus laevis: An Atlas of the Histology. A color look-up table helps to better distinguish between the … The Xenopus embryonic kidney, the pronephros, is a good model for studying kidney development and disease.